@prefix ex: <https://sparql.uniprot.org/.well-known/sparql-examples/> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix schema: <https://schema.org/> .
@prefix sh: <http://www.w3.org/ns/shacl#> .

ex:191_calcium_dependent_enzymes_with_binding_site a sh:SPARQLExecutable,
        sh:SPARQLSelectExecutable ;
    rdfs:comment "Find reviewed enzymes that both require Ca(2+) as a cofactor and have an annotated calcium-binding site - two distinct annotation types (a stated cofactor requirement vs. an explicit structural binding-site position) corroborating each other for the same ion. Both sides are matched by the actual ChEBI identity of calcium(2+) (CHEBI:29108), not by name text: up:cofactor points directly at the ChEBI IRI, while a binding site's up:ligand is an instance further typed via rdfs:subClassOf to the ChEBI class"@en ;
    sh:prefixes _:sparql_examples_prefixes ;
    sh:select """PREFIX up: <http://purl.uniprot.org/core/>
PREFIX rdfs: <http://www.w3.org/2000/01/rdf-schema#>
PREFIX CHEBI: <http://purl.obolibrary.org/obo/CHEBI_>

SELECT DISTINCT
  ?protein
WHERE {
  BIND(CHEBI:29108 AS ?calcium)
  ?protein up:reviewed true ;
    up:enzyme ?ec ;
    up:annotation ?cofactor ;
    up:annotation ?binding .
  ?cofactor a up:Cofactor_Annotation ;
    up:cofactor ?calcium .
  ?binding a up:Binding_Site_Annotation ;
    up:ligand ?ligand .
  ?ligand rdfs:subClassOf ?calcium .
}""" ;
    schema:keywords "calcium" , "cofactor" , "binding site" , "ChEBI" ;
    schema:target <https://sparql.uniprot.org/sparql/> .