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Sequencing technologies

Learning outcomes

After having completed this chapter you will be able to:

  • Describe the major applications of next generation sequencing
  • Reproduce the most frequently used sequencing methods
  • Describe the major steps taken during library preparation for Illumina sequencing
  • Explain why the length of the sequencing reads of Illumina sequencing are limited
  • Describe the general methods used for Oxford Nanopore Sequencing and PacBio sequencing


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Illumina sequencing by synthesis on YouTube

NEBnext library preparation poster