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Learning outcomes

After having completed this chapter you will be able to:

  • Illustrate the difference between short-read and long-read sequencing
  • Explain which type of invention led to development of long-read sequencing
  • Describe the basic techniques behind Oxford Nanopore sequencing and PacBio sequencing
  • Choose based on the characteristics of the discussed sequencing platforms which one is most suited for different situations


  • The introduction presentation:

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  • The sequencing technologies presentation:

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  • Nice review on long read sequencing in humans (also relevant for other species)
  • Review on long read sequencing data analysis