Introduction & cellranger

Learning outcomes

After having completed this chapter you will be able to:

• Explain what kind of information single cell RNA-seq can give you to answer a biological question
• Describe essential considerations during the design of a single cell RNA-seq experiment
• Describe the pros and cons of different single cell sequencing methods
• Use cellranger to:
  • To align reads and generate count tables
  • Perform basic QC on alignments and counts

Material

Introduction to scRNA-seq and techniques: