Analysis tools and QC
Learning outcomes
After having completed this chapter you will be able to:
- Load single cell data into R
- Explain the basic structure of a
Seurat
object and extract count data and metadata - Calculate and visualize quality measures based on:
- mitochondrial genes
- ribosomal genes
- hemoglobin genes
- relative gene expression
- Interpret the above quality measures per cell.
- Perform cell filtering based on quality thresholds