neXtProt

• 100 most-cited publications in neXtProt and the corresponding number of associated entries
• Authors who have reported more than 25000 human protein-protein interactions
• BRCA1 variants with at least 5 different Severe phenotypes
• Biological Process and Molecular Function GO terms related to UniPathway metabolic pathways
• Check there are no DNA binding region annotations with evidence from UniProt of quality SILVER
• Check there are no binding site annotations with evidence from UniProt of quality SILVER
• Check there are no coiled coil region annotations with evidence from UniProt of quality SILVER
• Check there are no compositionally biased region annotations with evidence from UniProt of quality SILVER
• Check there are no entries in the Cancer variants portal that do not have a variant phenotype annotation
• Check there are no entries in the Ion channels variants portal that do not have a variant phenotype annotation
• Check there are no entries in the Ion channels variants portal that do not have the GO molecular function Voltage-gated sodium channel activity (GO:0005248)
• Check there are no entries that do not have a gene name
• Check there are no entries that have a beginning position which is greater than the end position for the gene
• Check there are no entries that have a beginning position which is less than 0
• Check there are no entries that have an end position which is less than 0
• Check there are no entries where the isoform sequence starts with M and the variant at position 1 does not start with M
• Check there are no entries which are PE2-PE4 with a binary-interaction annotations with GOLD experimental evidence
• Check there are no entries which are PE2-PE4 with a crosslink annotation with GOLD experimental evidence which is NOT mass spectrometry from a source other than UniProt
• Check there are no entries which are PE2-PE4 with a disulfide bond annotation with GOLD experimental evidence which is NOT mass spectrometry from a source other than UniProt
• Check there are no entries which are PE2-PE4 with a glycosylation site annotation with GOLD experimental evidence which is NOT mass spectrometry from a source other than UniProt
• Check there are no entries which are PE2-PE4 with a lipidation site annotation with GOLD experimental evidence which is NOT mass spectrometry from a source other than UniProt
• Check there are no entries which are PE2-PE4 with a modified residue annotation with GOLD experimental evidence which is NOT mass spectrometry from a source other than UniProt
• Check there are no entries which are PE2-PE4 with a mutagenesis annotation with GOLD evidence assigned by neXtProt
• Check there are no entries which are PE2-PE4 with a ptm information annotation with positive GOLD experimental evidence from neXtProt and "phosphorylated" in the text
• Check there are no entries which are PE2-PE4 with an expression-info annotation of GOLD quality which has a description which contains "(at protein level)" and evidence assigned by neXtProt
• Check there are no entries which are PE3-PE4 with an expression-profile annotation with evidence from RNAseq and expression profile detected
• Check there are no entries which do not have a valid protein existence value
• Check there are no entries whose gene does not have a beginning position which has an end position
• Check there are no entries whose gene is located on a band which does NOT start with "p" or "q", is not "unknown" or null
• Check there are no entries whose gene is located on chromosome "unknown" for which the strand is NOT unknown
• Check there are no entries whose gene is located on chromosome "unknown" which have a band
• Check there are no entries whose gene is located on chromosome "unknown" which that have a beginning position which is NOT "0"
• Check there are no entries whose gene is located on chromosome "unknown" which that have a end position which is NOT "0"
• Check there are no entries whose gene is located on chromosome MT which have a band
• Check there are no entries whose gene is located on chromosome unknown which does not have AC NX_O00370 NX_Q96PT3 NX_Q96PT4 NX_Q9UN81
• Check there are no entries whose gene is not located either on chromosome 1-22, X, Y, MT, or unknown
• Check there are no entries whose gene is not located on the plus or minus strand or where the strand is unknown
• Check there are no entries whose gene is on chromosome unknown with a variant annotation with evidence from gnomAD
• Check there are no entries with binary interaction annotations from neXtProt for which the evidence code is NOT experimental evidence (ECO:0000006) or sequence similarity evidence used in manual assertion (ECO:0000250)
• Check there are no entries with DNA binding region annotations with evidence NOT from UniProt
• Check there are no entries with DNA binding region annotations with evidence from UniProt that is negative
• Check there are no entries with GO biological process annotations from neXtProt for which the evidence code is NOT experimental evidence (ECO:0000006) or sequence similarity evidence used in manual assertion (ECO:0000250)
• Check there are no entries with GO biological process annotations with evidence NOT from the GO Consortium or neXtProt
• Check there are no entries with GO biological process annotations with evidence from ARUK-UCL of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO biological process annotations with evidence from AgBase of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO biological process annotations with evidence from Alzheimers University of Toronto of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO biological process annotations with evidence from BHF-UCL of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO biological process annotations with evidence from CACAO of quality GOLD
• Check there are no entries with GO biological process annotations with evidence from CAFA of quality GOLD
• Check there are no entries with GO biological process annotations with evidence from DFLAT of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO biological process annotations with evidence from DIBU of the GO Consortium
• Check there are no entries with GO biological process annotations with evidence from ENSEMBL of quality GOLD
• Check there are no entries with GO biological process annotations with evidence from FlyBase of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO biological process annotations with evidence from GDB of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO biological process annotations with evidence from GO central of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO biological process annotations with evidence from GOC of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO biological process annotations with evidence from HGNC of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO biological process annotations with evidence from HGNC-UCL of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO biological process annotations with evidence from HPA of quality GOLD
• Check there are no entries with GO biological process annotations with evidence from IntAct of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO biological process annotations with evidence from InterPro of quality GOLD
• Check there are no entries with GO biological process annotations with evidence from LIFEdb of quality GOLD
• Check there are no entries with GO biological process annotations with evidence from MGI of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO biological process annotations with evidence from MTBbase of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO biological process annotations with evidence from NTNU SB of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO biological process annotations with evidence from PHI-base of the GO Consortium
• Check there are no entries with GO biological process annotations with evidence from PINC of quality GOLD
• Check there are no entries with GO biological process annotations with evidence from ParkinsonsUK-UCL of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO biological process annotations with evidence from Reactome of quality GOLD
• Check there are no entries with GO biological process annotations with evidence from Roslin Institute of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO biological process annotations with evidence from SGD of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO biological process annotations with evidence from SYSCILIA CCNET of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO biological process annotations with evidence from SynGO of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO biological process annotations with evidence from SynGO-UCL of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO biological process annotations with evidence from Uniprot of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO biological process annotations with evidence from WormBase of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO biological process annotations with evidence from YuBioLab of quality GOLD
• Check there are no entries with GO biological process annotations with evidence from dictyBase of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO biological process annotations with evidence having the evidence code HDA and the quality GOLD
• Check there are no entries with GO biological process annotations with evidence having the evidence code HEP and the quality GOLD
• Check there are no entries with GO biological process annotations with evidence having the evidence code HMP and the quality GOLD
• Check there are no entries with GO biological process annotations with evidence having the evidence code IBA and quality GOLD
• Check there are no entries with GO biological process annotations with evidence having the evidence code IEP and quality GOLD
• Check there are no entries with GO biological process annotations with the GO qualifier colocalizes_with
• Check there are no entries with GO biological process annotations with the GO qualifier contributes_to
• Check there are no entries with GO biological process annotations with the evidence code is ND (ECO:0000035)
• Check there are no entries with GO cellular component annotations from HPA for which the evidence code is NOT direct assay evidence used in manual assertion
• Check there are no entries with GO cellular component annotations from neXtProt for which the evidence code is NOT experimental evidence (ECO:0000006) or sequence similarity evidence used in manual assertion (ECO:0000250)
• Check there are no entries with GO cellular component annotations with evidence NOT from GO Consortium, HPA or neXtProt
• Check there are no entries with GO cellular component annotations with evidence from ARUK-UCL of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO cellular component annotations with evidence from AgBase of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO cellular component annotations with evidence from Alzheimers University of Toronto of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO cellular component annotations with evidence from BHF-UCL of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO cellular component annotations with evidence from CACAO of quality GOLD
• Check there are no entries with GO cellular component annotations with evidence from CAFA of quality GOLD
• Check there are no entries with GO cellular component annotations with evidence from DFLAT of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO cellular component annotations with evidence from DIBU of the GO Consortium
• Check there are no entries with GO cellular component annotations with evidence from ENSEMBL of quality GOLD
• Check there are no entries with GO cellular component annotations with evidence from FlyBase of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO cellular component annotations with evidence from GDB of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO cellular component annotations with evidence from GO central of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO cellular component annotations with evidence from GOC of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO cellular component annotations with evidence from HGNC of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO cellular component annotations with evidence from HGNC-UCL of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO cellular component annotations with evidence from HPA of quality GOLD
• Check there are no entries with GO cellular component annotations with evidence from IntAct of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO cellular component annotations with evidence from InterPro of quality GOLD
• Check there are no entries with GO cellular component annotations with evidence from LIFEdb of quality GOLD
• Check there are no entries with GO cellular component annotations with evidence from MGI of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO cellular component annotations with evidence from MTBbase of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO cellular component annotations with evidence from NTNU SB of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO cellular component annotations with evidence from PHI-base of the GO Consortium
• Check there are no entries with GO cellular component annotations with evidence from PINC of quality GOLD
• Check there are no entries with GO cellular component annotations with evidence from ParkinsonsUK-UCL of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO cellular component annotations with evidence from Reactome of quality GOLD
• Check there are no entries with GO cellular component annotations with evidence from Roslin Institute of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO cellular component annotations with evidence from SGD of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO cellular component annotations with evidence from SYSCILIA CCNET of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO cellular component annotations with evidence from SynGO of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO cellular component annotations with evidence from SynGO-UCL of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO cellular component annotations with evidence from Uniprot of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO cellular component annotations with evidence from WormBase of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO cellular component annotations with evidence from YuBioLab of quality GOLD
• Check there are no entries with GO cellular component annotations with evidence from dictyBase of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO cellular component annotations with evidence having the evidence code HDA and the quality GOLD
• Check there are no entries with GO cellular component annotations with evidence having the evidence code HEP and the quality GOLD
• Check there are no entries with GO cellular component annotations with evidence having the evidence code HMP and the quality GOLD
• Check there are no entries with GO cellular component annotations with evidence having the evidence code IBA and quality GOLD
• Check there are no entries with GO cellular component annotations with evidence having the evidence code IEP and quality GOLD
• Check there are no entries with GO cellular component annotations with the GO qualifier contributes_to
• Check there are no entries with GO cellular component annotations with the evidence code is ND (ECO:0000035)
• Check there are no entries with GO molecular function annotations from neXtProt for which the evidence code is NOT experimental evidence (ECO:0000006) or sequence similarity evidence used in manual assertion (ECO:0000250)
• Check there are no entries with GO molecular function annotations with evidence NOT from the GO Consortium or neXtProt
• Check there are no entries with GO molecular function annotations with evidence from ARUK-UCL of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO molecular function annotations with evidence from AgBase of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO molecular function annotations with evidence from Alzheimers University of Toronto of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO molecular function annotations with evidence from BHF-UCL of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO molecular function annotations with evidence from CACAO of quality GOLD
• Check there are no entries with GO molecular function annotations with evidence from CAFA of quality GOLD
• Check there are no entries with GO molecular function annotations with evidence from DFLAT of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO molecular function annotations with evidence from DIBU of the GO Consortium
• Check there are no entries with GO molecular function annotations with evidence from ENSEMBL of quality GOLD
• Check there are no entries with GO molecular function annotations with evidence from FlyBase of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO molecular function annotations with evidence from GDB of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO molecular function annotations with evidence from GO central of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO molecular function annotations with evidence from GOC of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO molecular function annotations with evidence from HGNC of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO molecular function annotations with evidence from HGNC-UCL of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO molecular function annotations with evidence from HPA of quality GOLD
• Check there are no entries with GO molecular function annotations with evidence from IntAct of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO molecular function annotations with evidence from InterPro of quality GOLD
• Check there are no entries with GO molecular function annotations with evidence from LIFEdb of quality GOLD
• Check there are no entries with GO molecular function annotations with evidence from MGI of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO molecular function annotations with evidence from MTBbase of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO molecular function annotations with evidence from NTNU SB of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO molecular function annotations with evidence from PHI-base of the GO Consortium
• Check there are no entries with GO molecular function annotations with evidence from PINC of quality GOLD
• Check there are no entries with GO molecular function annotations with evidence from ParkinsonsUK-UCL of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO molecular function annotations with evidence from Reactome of quality GOLD
• Check there are no entries with GO molecular function annotations with evidence from Roslin Institute of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO molecular function annotations with evidence from SGD of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO molecular function annotations with evidence from SYSCILIA CCNET of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO molecular function annotations with evidence from SynGO of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO molecular function annotations with evidence from SynGO-UCL of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO molecular function annotations with evidence from Uniprot of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO molecular function annotations with evidence from WormBase of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO molecular function annotations with evidence from YuBioLab of quality GOLD
• Check there are no entries with GO molecular function annotations with evidence from dictyBase of quality SILVER and evidence code not IBA, IEP, HMP, HDA or HEP
• Check there are no entries with GO molecular function annotations with evidence having the evidence code HDA and quality GOLD
• Check there are no entries with GO molecular function annotations with evidence having the evidence code HEP and quality GOLD
• Check there are no entries with GO molecular function annotations with evidence having the evidence code HMP and quality GOLD
• Check there are no entries with GO molecular function annotations with evidence having the evidence code IBA and quality GOLD
• Check there are no entries with GO molecular function annotations with evidence having the evidence code IEP and quality GOLD
• Check there are no entries with GO molecular function annotations with the GO qualifier colocalizes_with
• Check there are no entries with GO molecular function annotations with the evidence code is ND (ECO:0000035)
• Check there are no entries with PDB mapping annotation and no xref to PDB
• Check there are no entries with PDB mapping annotation and no xref to PDBsum
• Check there are no entries with PDB mapping annotations with evidence NOT from UniProt
• Check there are no entries with PDB mapping annotations with evidence from UniProt that is negative
• Check there are no entries with PDB mapping annotations with evidence from Uniprot of quality SILVER
• Check there are no entries with PTM info annotations from neXtProt for which the evidence code is NOT experimental evidence or sequence similarity evidence used in manual assertion
• Check there are no entries with PTM information annotations with evidence NOT from UniProt or neXtProt
• Check there are no entries with PTM information annotations with evidence from UniProt that is negative
• Check there are no entries with PTM information annotations with evidence from Uniprot of quality SILVER
• Check there are no entries with S-nitrosylation annotations that do not have the keyword S-nitrosylation (KW-0702)
• Check there are no entries with SRM peptide mapping annotations from SRMAtlas for which the evidence code is NOT mass spectrometry evidence
• Check there are no entries with SRM peptide mapping annotations with evidence NOT from SRMAtlas
• Check there are no entries with SRM peptide mapping annotations with evidence from SRMAtlas of quality SILVER
• Check there are no entries with SRM peptide mapping annotations with evidence from UniProt that is negative
• Check there are no entries with UniProt keyword annotations with evidence NOT from UniProt
• Check there are no entries with UniProt keyword annotations with evidence from UniProt that is negative
• Check there are no entries with UniProt keyword annotations with evidence from Uniprot of quality SILVER
• Check there are no entries with a PTM on a non-proteotypic peptide (excluding PTMs with evidence from UniProt)
• Check there are no entries with a binary interaction annotation from ENYO with no publication in evidence
• Check there are no entries with a gene name which does not contain a capital letter or is not "unknown"
• Check there are no entries with a keyword in the category "Biological process" that does not apply to human proteins
• Check there are no entries with a keyword in the category "Cellular component" that does not apply to human proteins
• Check there are no entries with a keyword in the category "Coding sequence diversity" that does not apply to human proteins
• Check there are no entries with a keyword in the category "Developmental stage" that does not apply to human proteins
• Check there are no entries with a keyword in the category "Disease" that does not apply to human proteins
• Check there are no entries with a keyword in the category "Domain" that does not apply to human proteins
• Check there are no entries with a keyword in the category "Ligand" that does not apply to human proteins
• Check there are no entries with a keyword in the category "Molecular function" that does not apply to human proteins
• Check there are no entries with a keyword in the category "Post-translational modification" that does not apply to human proteins
• Check there are no entries with a keyword in the category "Technical term" that does not apply to human proteins
• Check there are no entries with a localization keyword that do not have the corresponding subcellular location annotation and/or GO cellular component annotatio
• Check there are no entries with a modified residue annotation specifying an ADP-ribosylation site that do not have the keyword ADP-ribosylation (KW-0013)
• Check there are no entries with a modified residue annotation specifying an acetylation site that do not have the keyword Acetylation (KW-0007)
• Check there are no entries with a natural peptide from MassIVE with an AC which does NOT start with "MSVp"
• Check there are no entries with a natural peptide from PeptideAtlas with an AC which does NOT start with "Pap"
• Check there are no entries with a peptide mapping annotation with evidence from MassIVE but no xref to MassIVE
• Check there are no entries with a peptide mapping annotation with evidence from PeptideAtlas but no xref to PeptideAtlas
• Check there are no entries with a ptm info annotation referring to ADP-ribosylation that do not have the keyword ADP-ribosylation (KW-0013)
• Check there are no entries with a ptm info annotation referring to S-nitrosylation that do not have the keyword S-nitrosylation (KW-0702)
• Check there are no entries with a ptm info annotation referring to acetylation that do not have the keyword Acetylation (KW-0007)
• Check there are no entries with a ptm info annotation referring to glycosylation that do not have the keyword Glycoprotein (KW-0325)
• Check there are no entries with a ptm info annotation referring to methylation that do not have the keyword Methylation (KW-0488)
• Check there are no entries with a ptm info annotation referring to phosphorylation that do not have the keyword Phosphoprotein (KW-0597)
• Check there are no entries with a subcellular location annotation that do not have the corresponding keyword
• Check there are no entries with a synthetic peptide from SRMAtlas with an AC which does NOT start with "Pap"
• Check there are no entries with a variant annotation having a xref starting with "rs" that do not have the keyword KW-0621 (Polymorphism)
• Check there are no entries with a variant annotation with an allele count of 0
• Check there are no entries with a variant annotation with an allele frequency of 0
• Check there are no entries with a variant annotation with an allele number of 0
• Check there are no entries with a variant annotation with an xref starting with COSM that do not have evidence from COSMIC
• Check there are no entries with a variant annotation with an xref starting with rs that do not have evidence from dbSNP or UniProt
• Check there are no entries with a variant annotation with evidence from COSMIC that do not have an xref starting with COSM
• Check there are no entries with a variant annotation with evidence from dbSNP that does not have an xref starting with rs
• Check there are no entries with a variant annotation with evidence from gnomAD that do not have a homozygote count
• Check there are no entries with a variant annotation with evidence from gnomAD that do not have an allele count
• Check there are no entries with a variant annotation with evidence from gnomAD that do not have an allele frequency
• Check there are no entries with a variant annotation with evidence from gnomAD that do not have an allele number
• Check there are no entries with a variant annotation with no original value
• Check there are no entries with a variant annotation with the end greater than the sequence length
• Check there are no entries with a variant annotation with the end less than 1
• Check there are no entries with a variant annotation with the end less than the start
• Check there are no entries with a variant annotation with the start greater than the sequence length
• Check there are no entries with a variant annotation with the start less than 1
• Check there are no entries with a variant, responsible for a disease, that do not have the keyword KW-0225 (Disease mutation)
• Check there are no entries with absorption maximum annotations with evidence NOT from UniProt
• Check there are no entries with absorption maximum annotations with evidence from UniProt of quality SILVER
• Check there are no entries with absorption maximum annotations with evidence from UniProt that is negative
• Check there are no entries with absorption note annotations with evidence NOT from UniProt
• Check there are no entries with absorption note annotations with evidence from UniProt of quality SILVER
• Check there are no entries with absorption note annotations with evidence from UniProt that is negative
• Check there are no entries with active site annotations with evidence NOT from UniProt
• Check there are no entries with active site annotations with evidence from UniProt of quality SILVER
• Check there are no entries with active site annotations with evidence from UniProt that is negative
• Check there are no entries with activity regulation (previously enzyme regulation) annotations with evidence NOT from UniProt
• Check there are no entries with activity regulation (previously enzyme regulation) annotations with evidence from UniProt of quality SILVER
• Check there are no entries with activity regulation (previously enzyme regulation) annotations with evidence from UniProt that is negative
• Check there are no entries with allergen annotations with evidence NOT from UniProt
• Check there are no entries with allergen annotations with evidence from UniProt of quality SILVER
• Check there are no entries with allergen annotations with evidence from UniProt that is negative
• Check there are no entries with an SRM peptide mapping annotation with evidence from SRMAtlas but no xref to SRMAtlas
• Check there are no entries with an interaction mapping annotation from ENYO with no publication in evidence
• Check there are no entries with an xref to PDBsum and no PDB mapping annotation
• Check there are no entries with an xref to SRMAtlas but no SRM peptide mapping annotation with evidence from SRMAtlas
• Check there are no entries with an xref to an ENSP but no xref to an ENSG
• Check there are no entries with an xref to an ENSP but no xref to an ENST
• Check there are no entries with antibody mapping annotations and no xrefs from HPA
• Check there are no entries with antibody mapping annotations for which the antibody AC does NOT start with "HPA"
• Check there are no entries with antibody mapping annotations from HPA for which the evidence code is NOT heterologous protein expression evidence
• Check there are no entries with antibody mapping annotations from HPA for which there is xref from HPA
• Check there are no entries with antibody mapping annotations with evidence NOT from HPA
• Check there are no entries with antibody mapping annotations with evidence from HPA of quality SILVER
• Check there are no entries with antibody mapping annotations with evidence from HPA that is negative
• Check there are no entries with beta strand annotations with evidence NOT from UniProt
• Check there are no entries with beta strand annotations with evidence from UniProt of quality SILVER
• Check there are no entries with beta strand annotations with evidence from UniProt that is negative
• Check there are no entries with binary interaction annotations from IntAct for which the evidence code is NOT physical interaction evidence used in manual assertion
• Check there are no entries with binary interaction annotations with evidence NOT from ENYO, IntAct or neXtProt
• Check there are no entries with binary interaction annotations with evidence from ENYO for which the evidence code is NOT physical interaction evidence used in manual assertion (ECO:0000353)
• Check there are no entries with binary interaction annotations with evidence from ENYO of quality SILVER
• Check there are no entries with binary interaction annotations with evidence from ENYO that is negative
• Check there are no entries with binary interaction annotations with evidence from IntAct that is negative
• Check there are no entries with binding site annotations with evidence NOT from UniProt
• Check there are no entries with binding site annotations with evidence from UniProt that is negative
• Check there are no entries with calcium binding region annotations with evidence NOT from UniProt
• Check there are no entries with calcium binding region annotations with evidence from UniProt of quality SILVER
• Check there are no entries with calcium binding region annotations with evidence from UniProt that is negative
• Check there are no entries with catalytic activity annotations with evidence NOT from UniProt
• Check there are no entries with catalytic activity annotations with evidence from UniProt of quality SILVER
• Check there are no entries with catalytic activity annotations with evidence from UniProt that is negative
• Check there are no entries with caution annotations with evidence NOT from UniProt
• Check there are no entries with caution annotations with evidence from UniProt of quality SILVER
• Check there are no entries with caution annotations with evidence from UniProt that is negative
• Check there are no entries with cleavage site annotations with evidence NOT from UniProt
• Check there are no entries with cleavage site annotations with evidence from UniProt of quality SILVER
• Check there are no entries with cleavage site annotations with evidence from UniProt that is negative
• Check there are no entries with cofactor annotations with evidence NOT from UniProt
• Check there are no entries with cofactor annotations with evidence from UniProt of quality SILVER
• Check there are no entries with cofactor annotations with evidence from UniProt that is negative
• Check there are no entries with cofactor information annotations with evidence NOT from UniProt
• Check there are no entries with cofactor information annotations with evidence from UniProt of quality SILVER
• Check there are no entries with cofactor information annotations with evidence from UniProt that is negative
• Check there are no entries with coiled coil region annotations with evidence NOT from UniProt
• Check there are no entries with coiled coil region annotations with evidence from UniProt that is negative
• Check there are no entries with compositionally biased region annotations with evidence NOT from UniProt
• Check there are no entries with compositionally biased region annotations with evidence from UniProt that is negative
• Check there are no entries with cross-link annotations with evidence from UniProt that is negative
• Check there are no entries with cross-link annotations with evidence from neXtProt that is negative
• Check there are no entries with crosslink annotations from neXtProt for which the evidence code is NOT mass spectrometry evidence
• Check there are no entries with crosslink annotations that do not have the keyword Isopeptide bond (KW-1017)
• Check there are no entries with crosslink annotations with evidence NOT from UniProt or neXtProt
• Check there are no entries with crosslink annotations with evidence from UniProt of quality SILVER
• Check there are no entries with developmental stage information annotations with evidence NOT from UniProt
• Check there are no entries with developmental stage information annotations with evidence from UniProt of quality SILVER
• Check there are no entries with developmental stage information annotations with evidence from UniProt that is negative
• Check there are no entries with disease annotations from ORPHANET for which the evidence code is NOT curator inference used in manual assertion
• Check there are no entries with disease annotations with evidence NOT from neXtProt, Orphanet or UniProt
• Check there are no entries with disease annotations with evidence from Orphanet of quality SILVER
• Check there are no entries with disease annotations with evidence from Orphanet that is negative
• Check there are no entries with disease annotations with evidence from UniProt of quality SILVER
• Check there are no entries with disease annotations with evidence from UniProt that is negative
• Check there are no entries with disulfide bond annotations with evidence NOT from UniProt
• Check there are no entries with disulfide bond annotations with evidence from UniProt of quality SILVER
• Check there are no entries with disulfide bond annotations with evidence from UniProt that is negative
• Check there are no entries with domain annotations with evidence NOT from UniProt
• Check there are no entries with domain annotations with evidence from UniProt of quality SILVER
• Check there are no entries with domain annotations with evidence from UniProt that is negative
• Check there are no entries with domain information annotations with evidence NOT from UniProt
• Check there are no entries with domain information annotations with evidence from UniProt of quality SILVER
• Check there are no entries with domain information annotations with evidence from UniProt that is negative
• Check there are no entries with electrophysiological parameter annotations from neXtProt for which the evidence code is NOT experimental evidence (ECO:0000006) or sequence similarity evidence used in manual assertion (ECO:0000250)
• Check there are no entries with electrophysiological parameter annotations with evidence NOT from neXtProt
• Check there are no entries with electrophysiological parameter annotations with evidence from neXtProt that is negative
• Check there are no entries with enzyme classification annotations with evidence NOT from UniProt
• Check there are no entries with enzyme classification annotations with evidence from UniProt of quality SILVER
• Check there are no entries with enzyme classification annotations with evidence from UniProt that is negative
• Check there are no entries with expression information annotations from HPA for which the evidence code is NOT immunohistochemistry evidence, RNA-sequencing evidence or single-cell RNA-sequencing evidence
• Check there are no entries with expression information annotations with evidence NOT from UniProt, HPA or neXtProt
• Check there are no entries with expression information annotations with evidence from HPA that is negative
• Check there are no entries with expression information annotations with evidence from UniProt of quality SILVER
• Check there are no entries with expression information annotations with evidence from UniProt that is negative
• Check there are no entries with expression information annotations with evidence from neXtProt that is negative
• Check there are no entries with expression profile annotations from Bgee for which the evidence code is NOT microarray RNA expression level evidence (ECO:0000104), transcript expression evidence (ECO:0000009) or RNA-sequencing evidence (ECO:0000295)
• Check there are no entries with expression profile annotations from HPA for which the evidence code is NOT immunohistochemistry evidence, RNA-seq evidence or single-cell RNA-sequencing evidence
• Check there are no entries with expression profile annotations with RNA-seq evidence (ECO:0000295) from HPA which is SILVER
• Check there are no entries with expression profile annotations with evidence NOT from Bgee or HPA
• Check there are no entries with expression profile annotations with evidence from Bgee to an anatomy term which is different from the anatomy term in the experimental context
• Check there are no entries with expression profile annotations with evidence from EST which are NOT from Bgee
• Check there are no entries with expression profile annotations with evidence from EST with an expression level other than detected
• Check there are no entries with expression profile annotations with evidence from HPA to an anatomy term which is different from the anatomy term in the experimental context
• Check there are no entries with expression profile annotations with evidence from IHC which are NOT from HPA
• Check there are no entries with expression profile annotations with evidence from IHC with an expression level other than high, medium, low or negative
• Check there are no entries with expression profile annotations with evidence from RNA-seq which are NOT from HPA or Bgee
• Check there are no entries with expression profile annotations with evidence from RNA-seq with an expression level other than detected or not detected
• Check there are no entries with expression profile annotations with evidence from microarray which are NOT from Bgee
• Check there are no entries with expression profile annotations with evidence from microarray with an expression level other than detected or not detected
• Check there are no entries with family annotations with evidence NOT from UniProt
• Check there are no entries with family annotations with evidence from UniProt of quality SILVER
• Check there are no entries with family annotations with evidence from UniProt that is negative
• Check there are no entries with function information annotations with evidence NOT from UniProt
• Check there are no entries with function information annotations with evidence from UniProt of quality SILVER
• Check there are no entries with function information annotations with evidence from UniProt that is negative
• Check there are no entries with glycosylation site annotations from GlyConnect for which the evidence code is NOT experimental evidence used in manual assertion
• Check there are no entries with glycosylation site annotations from neXtProt for which the evidence code is NOT mass spectrometry evidence
• Check there are no entries with glycosylation site annotations that do not have the keyword Glycoprotein (KW-0325)
• Check there are no entries with glycosylation site annotations with evidence NOT from UniProt, neXtProt or GlyConnect
• Check there are no entries with glycosylation site annotations with evidence from UniProt that is negative
• Check there are no entries with glycosylation site annotations with evidence from Uniprot of quality SILVER
• Check there are no entries with glycosylation site annotations with evidence from neXtProt that is negative
• Check there are no entries with helix annotations with evidence NOT from UniProt
• Check there are no entries with helix annotations with evidence from UniProt of quality SILVER
• Check there are no entries with helix annotations with evidence from UniProt that is negative
• Check there are no entries with induction annotations with evidence NOT from UniProt
• Check there are no entries with induction annotations with evidence from UniProt of quality SILVER
• Check there are no entries with induction annotations with evidence from UniProt that is negative
• Check there are no entries with initiator methionine annotations with evidence NOT from UniProt
• Check there are no entries with initiator methionine annotations with evidence from UniProt of quality SILVER
• Check there are no entries with initiator methionine annotations with evidence from UniProt that is negative
• Check there are no entries with interacting region annotations with evidence NOT from UniProt
• Check there are no entries with interacting region annotations with evidence from UniProt of quality SILVER
• Check there are no entries with interacting region annotations with evidence from UniProt that is negative
• Check there are no entries with interaction information annotations with evidence NOT from UniProt
• Check there are no entries with interaction information annotations with evidence from UniProt of quality SILVER
• Check there are no entries with interaction information annotations with evidence from UniProt that is negative
• Check there are no entries with interaction mapping annotations with evidence NOT from ENYO
• Check there are no entries with interaction mapping annotations with evidence from ENYO for which the evidence code is NOT physical interaction evidence used in manual assertion (ECO:0000353)
• Check there are no entries with interaction mapping annotations with evidence from ENYO of quality SILVER
• Check there are no entries with interaction mapping annotations with evidence from ENYO that is negative
• Check there are no entries with intramembrane region annotations with evidence NOT from UniProt
• Check there are no entries with intramembrane region annotations with evidence from UniProt of quality SILVER
• Check there are no entries with intramembrane region annotations with evidence from UniProt that is negative
• Check there are no entries with keywords corresponding to keyword categories
• Check there are no entries with kinetic KM annotations with evidence NOT from UniProt
• Check there are no entries with kinetic KM annotations with evidence from UniProt of quality SILVER
• Check there are no entries with kinetic KM annotations with evidence from UniProt that is negative
• Check there are no entries with kinetic Vmax annotations with evidence NOT from UniProt
• Check there are no entries with kinetic Vmax annotations with evidence from UniProt of quality SILVER
• Check there are no entries with kinetic Vmax annotations with evidence from UniProt that is negative
• Check there are no entries with kinetic note annotations with evidence NOT from UniProt
• Check there are no entries with kinetic note annotations with evidence from UniProt of quality SILVER
• Check there are no entries with kinetic note annotations with evidence from UniProt that is negative
• Check there are no entries with lipidation site annotations with evidence NOT from UniProt
• Check there are no entries with lipidation site annotations with evidence from UniProt of quality SILVER
• Check there are no entries with lipidation site annotations with evidence from UniProt that is negative
• Check there are no entries with mature protein annotations with evidence NOT from UniProt
• Check there are no entries with mature protein annotations with evidence from UniProt of quality SILVER
• Check there are no entries with mature protein annotations with evidence from UniProt that is negative
• Check there are no entries with metal binding site annotations with evidence NOT from UniProt
• Check there are no entries with metal binding site annotations with evidence from UniProt of quality SILVER
• Check there are no entries with metal binding site annotations with evidence from UniProt that is negative
• Check there are no entries with methylation annotations that do not have the keyword Methylation (KW-0488)
• Check there are no entries with miscellaneous annotations with evidence NOT from UniProt
• Check there are no entries with miscellaneous annotations with evidence from UniProt of quality SILVER
• Check there are no entries with miscellaneous annotations with evidence from UniProt that is negative
• Check there are no entries with miscellaneous region annotations with evidence NOT from UniProt
• Check there are no entries with miscellaneous region annotations with evidence from UniProt of quality SILVER
• Check there are no entries with miscellaneous region annotations with evidence from UniProt that is negative
• Check there are no entries with miscellaneous site annotations with evidence NOT from UniProt
• Check there are no entries with miscellaneous site annotations with evidence from UniProt of quality SILVER
• Check there are no entries with miscellaneous site annotations with evidence from UniProt that is negative
• Check there are no entries with mitochondrial transit peptide annotations with evidence NOT from UniProt
• Check there are no entries with mitochondrial transit peptide annotations with evidence from UniProt of quality SILVER
• Check there are no entries with mitochondrial transit peptide annotations with evidence from UniProt that is negative
• Check there are no entries with modified residue annotations from PeptideAtlas Phosphoproteome for which the evidence code is NOT mass spectrometry evidence
• Check there are no entries with modified residue annotations from neXtProt for which the evidence code is NOT mass spectrometry evidence, experimental evidence or sequence similarity evidence used in manual assertion
• Check there are no entries with modified residue annotations with evidence NOT from UniProt, neXtProt or PeptideAtlas Phosphoproteome
• Check there are no entries with modified residue annotations with evidence from PeptideAtlas Phosphoproteome that is negative
• Check there are no entries with modified residue annotations with evidence from UniProt of quality SILVER
• Check there are no entries with modified residue annotations with evidence from UniProt that is negative
• Check there are no entries with modified residue annotations with evidence from neXtProt that is negative
• Check there are no entries with modified residue phosphorylation site annotations that do not have the keyword Phosphoprotein (KW-0597)
• Check there are no entries with mutagenesis annotations from neXtProt for which the evidence code is NOT experimental evidence used in manual assertion
• Check there are no entries with mutagenesis annotations with evidence NOT from neXtProt or UniProt
• Check there are no entries with mutagenesis annotations with evidence from UniProt of quality SILVER
• Check there are no entries with mutagenesis annotations with evidence from UniProt that is negative
• Check there are no entries with mutagenesis annotations with evidence from neXtProt of quality SILVER
• Check there are no entries with mutagenesis annotations with evidence from neXtProt that is negative
• Check there are no entries with no Swiss-Prot keyword
• Check there are no entries with no chromosome location
• Check there are no entries with non-compliant glycosylation sites from GlyConnect
• Check there are no entries with non-compliant glycosylation sites from UniProt
• Check there are no entries with non-compliant glycosylation sites from neXtProt
• Check there are no entries with nonconsecutive residue annotations with evidence NOT from UniProt
• Check there are no entries with nonconsecutive residue annotations with evidence from UniProt of quality SILVER
• Check there are no entries with nonconsecutive residue annotations with evidence from UniProt that is negative
• Check there are no entries with nonterminal residue annotations with evidence NOT from UniProt
• Check there are no entries with nonterminal residue annotations with evidence from UniProt of quality SILVER
• Check there are no entries with nonterminal residue annotations with evidence from UniProt that is negative
• Check there are no entries with nucleotide phosphate binding region annotations with evidence NOT from UniProt
• Check there are no entries with nucleotide phosphate binding region annotations with evidence from UniProt of quality SILVER
• Check there are no entries with nucleotide phosphate binding region annotations with evidence from UniProt that is negative
• Check there are no entries with pH dependence annotations with evidence NOT from UniProt
• Check there are no entries with pH dependence annotations with evidence from UniProt that is negative
• Check there are no entries with pH dependence annotations with evidence from Uniprot of quality SILVER
• Check there are no entries with pathway annotations from KEGG Pathway for which the evidence code is NOT curator inference used in manual assertion
• Check there are no entries with pathway annotations from Reactome for which the evidence code is NOT curator inference used in manual assertion
• Check there are no entries with pathway annotations with evidence NOT from KEGG Pathway, Reactome or UniProt
• Check there are no entries with pathway annotations with evidence from KEGG Pathway of quality SILVER
• Check there are no entries with pathway annotations with evidence from KEGG Pathway that is negative
• Check there are no entries with pathway annotations with evidence from Reactome of quality SILVER
• Check there are no entries with pathway annotations with evidence from Reactome that is negative
• Check there are no entries with pathway annotations with evidence from UniProt that is negative
• Check there are no entries with pathway annotations with evidence from Uniprot of quality SILVER
• Check there are no entries with peptide mapping annotations from MassIVE for which the evidence code is NOT mass spectrometry evidence
• Check there are no entries with peptide mapping annotations from neXtProt (MDATA) for which the evidence code is NOT mass spectrometry evidence
• Check there are no entries with peptide mapping annotations from neXtProt for which the evidence code is NOT mass spectrometry evidence
• Check there are no entries with peptide mapping annotations with evidence NOT from MassIVE, PeptideAtlas or neXtProt
• Check there are no entries with peptide mapping annotations with evidence from MassIVE of quality SILVER
• Check there are no entries with peptide mapping annotations with evidence from MassIVE that is negative
• Check there are no entries with peptide mapping annotations with evidence from PeptideAtlas of quality SILVER
• Check there are no entries with peptide mapping annotations with evidence from PeptideAtlas that is negative
• Check there are no entries with peptide mapping annotations with evidence from neXtProt of quality SILVER
• Check there are no entries with peptide mapping annotations with evidence from neXtProt that is negative
• Check there are no entries with peptide mapping annotations with peptides of length 6 aa or less
• Check there are no entries with peroxisome transit peptide annotations with evidence NOT from UniProt
• Check there are no entries with peroxisome transit peptide annotations with evidence from UniProt that is negative
• Check there are no entries with peroxisome transit peptide annotations with evidence from Uniprot of quality SILVER
• Check there are no entries with pharmaceutical annotations with evidence NOT from UniProt
• Check there are no entries with pharmaceutical annotations with evidence from UniProt that is negative
• Check there are no entries with pharmaceutical annotations with evidence from Uniprot of quality SILVER
• Check there are no entries with phenotypic variation annotations from neXtProt for which the evidence code is NOT experimental evidence (ECO:0000006) or sequence similarity evidence used in manual assertion (ECO:0000250)
• Check there are no entries with phenotypic variation annotations with evidence NOT from neXtProt
• Check there are no entries with phenotypic variation annotations with evidence from neXtProt that is negative
• Check there are no entries with propeptide annotations with evidence NOT from UniProt
• Check there are no entries with propeptide annotations with evidence from UniProt that is negative
• Check there are no entries with propeptide annotations with evidence from Uniprot of quality SILVER
• Check there are no entries with redox potential annotations with evidence NOT from UniProt
• Check there are no entries with redox potential annotations with evidence from UniProt that is negative
• Check there are no entries with redox potential annotations with evidence from Uniprot of quality SILVER
• Check there are no entries with repeat annotations with evidence NOT from UniProt
• Check there are no entries with repeat annotations with evidence from UniProt that is negative
• Check there are no entries with repeat annotations with evidence from Uniprot of quality SILVER
• Check there are no entries with selenocysteine annotations with evidence NOT from UniProt
• Check there are no entries with selenocysteine annotations with evidence from UniProt that is negative
• Check there are no entries with selenocysteine annotations with evidence from Uniprot of quality SILVER
• Check there are no entries with sequence caution annotations with evidence NOT from UniProt
• Check there are no entries with sequence caution annotations with evidence from UniProt that is negative
• Check there are no entries with sequence caution annotations with evidence from Uniprot of quality SILVER
• Check there are no entries with sequence conflict annotations with evidence NOT from UniProt
• Check there are no entries with sequence conflict annotations with evidence from UniProt that is negative
• Check there are no entries with sequence conflict annotations with evidence from Uniprot of quality SILVER
• Check there are no entries with short sequence motif annotations with evidence NOT from UniProt
• Check there are no entries with short sequence motif annotations with evidence from UniProt that is negative
• Check there are no entries with short sequence motif annotations with evidence from Uniprot of quality SILVER
• Check there are no entries with signal peptide annotations with evidence NOT from UniProt
• Check there are no entries with signal peptide annotations with evidence from UniProt that is negative
• Check there are no entries with signal peptide annotations with evidence from Uniprot of quality SILVER
• Check there are no entries with small molecule interaction annotations from DrugBank for which the evidence code is NOT curator inference used in manual assertion
• Check there are no entries with small molecule interaction annotations with evidence NOT from DrugBank or UniProt
• Check there are no entries with small molecule interaction annotations with evidence from DrugBank of quality GOLD
• Check there are no entries with small molecule interaction annotations with evidence from DrugBank that is negative
• Check there are no entries with small molecule interaction annotations with evidence from UniProt that is negative
• Check there are no entries with small molecule interaction annotations with evidence from Uniprot of quality SILVER
• Check there are no entries with subcellular location annotations from Dyp for which the evidence code is NOT reporter gene assay evidence
• Check there are no entries with subcellular location annotations from GFP-cDNAEMBL for which the evidence code is NOT reporter gene assay evidence
• Check there are no entries with subcellular location annotations from HPA for which the evidence code is NOT immunocytochemistry evidence
• Check there are no entries with subcellular location annotations with evidence NOT from UniProt, HPA, Dyp or GFP-cDNAEMBL
• Check there are no entries with subcellular location annotations with evidence from Dyp of quality GOLD
• Check there are no entries with subcellular location annotations with evidence from Dyp that is negative
• Check there are no entries with subcellular location annotations with evidence from GFP-cDNA@EMBL of quality GOLD
• Check there are no entries with subcellular location annotations with evidence from GFP-cDNAEMBL that is negative
• Check there are no entries with subcellular location annotations with evidence from HPA that is negative
• Check there are no entries with subcellular location annotations with evidence from UniProt that is negative
• Check there are no entries with subcellular location annotations with evidence from Uniprot of quality SILVER
• Check there are no entries with subcellular location note annotations from HPA for which there is no subcellular location annotation from HPA
• Check there are no entries with subcellular location note annotations with evidence NOT from UniProt
• Check there are no entries with subcellular location note annotations with evidence from UniProt that is negative
• Check there are no entries with subcellular location note annotations with evidence from Uniprot of quality SILVER
• Check there are no entries with temperature dependence annotations with evidence NOT from UniProt
• Check there are no entries with temperature dependence annotations with evidence from UniProt that is negative
• Check there are no entries with temperature dependence annotations with evidence from Uniprot of quality SILVER
• Check there are no entries with the keyword KW-0225 (Disease mutation) that do not have a variant responsible for a disease
• Check there are no entries with the keyword KW-0621 (Polymorphism) that do not have a variant annotation with evidence from UniProt or dbSNP and having a xref starting with "rs"
• Check there are no entries with topological domain annotations with evidence NOT from UniProt
• Check there are no entries with topological domain annotations with evidence from UniProt that is negative
• Check there are no entries with topological domain annotations with evidence from Uniprot of quality SILVER
• Check there are no entries with transmembrane region annotations with evidence NOT from UniProt
• Check there are no entries with transmembrane region annotations with evidence from UniProt that is negative
• Check there are no entries with transmembrane region annotations with evidence from Uniprot of quality SILVER
• Check there are no entries with transport activity annotations from TCDB for which the evidence code is NOT curator inference used in manual assertion
• Check there are no entries with transport activity annotations with evidence NOT from TCDB
• Check there are no entries with transport activity annotations with evidence from TCDB of quality SILVER
• Check there are no entries with transport activity annotations with evidence from TCDB that is negative
• Check there are no entries with turn annotations with evidence NOT from UniProt
• Check there are no entries with turn annotations with evidence from UniProt that is negative
• Check there are no entries with turn annotations with evidence from Uniprot of quality SILVER
• Check there are no entries with variant annotations from Cosmic for which the evidence code is NOT nucleotide sequencing assay evidence
• Check there are no entries with variant annotations from gnomAD for which the evidence code is NOT nucleotide sequencing assay evidence
• Check there are no entries with variant annotations from neXtProt for which the evidence code is NOT nucleotide sequencing assay evidence (ECO:0000219) or sequence similarity evidence used in manual assertion (ECO:0000250)
• Check there are no entries with variant annotations with evidence NOT from Cellosaurus, Cosmic, dbSNP, gnomAD, neXtProt or UniProt
• Check there are no entries with variant annotations with evidence from COSMIC of quality GOLD
• Check there are no entries with variant annotations with evidence from COSMIC which are synonymous
• Check there are no entries with variant annotations with evidence from Cosmic that is negative
• Check there are no entries with variant annotations with evidence from UniProt that is negative
• Check there are no entries with variant annotations with evidence from Uniprot of quality SILVER
• Check there are no entries with variant annotations with evidence from dbSNP and quality silver for which the evidence code is NOT nucleotide sequencing assay evidence
• Check there are no entries with variant annotations with evidence from dbSNP that is negative
• Check there are no entries with variant annotations with evidence from dbSNP which are synonymous
• Check there are no entries with variant annotations with evidence from gnomAD of quality SILVER
• Check there are no entries with variant annotations with evidence from gnomAD that is negative
• Check there are no entries with variant annotations with evidence from neXtProt of quality SILVER
• Check there are no entries with variant annotations with evidence from neXtProt that is negative
• Check there are no entries with variant information annotations with evidence NOT from UniProt
• Check there are no entries with variant information annotations with evidence from UniProt that is negative
• Check there are no entries with variant information annotations with evidence from Uniprot of quality SILVER
• Check there are no entries with zinc finger region annotations with evidence NOT from UniProt
• Check there are no entries with zinc finger region annotations with evidence from Uniprot of quality SILVER
• Check there are no entries with zinc finger region with evidence from UniProt that is negative
• Check there are no non-standard amino acid terms with no entry associated
• Check there are no organelle terms with no entry associated
• Check there are no protein domains with no entry associated
• Check there are no protein families with no entry associated
• Check there are no protein modification effect terms with no entry associated
• Check there are no topological domains with no entry associated
• Diseases/phenotypes associated with coding variants and associated publications for a given gene
• Distribution of interaction detection methods for each year (ENYO data)
• Domains that are entirely covered by 3D structures
• Frequent variants at a PTM site
• GO_MF terms associated with proteins binding estradiol and/or similar molecules (substructure search with SMILES)
• Glycosylation sites and cross links positions on SwissProt canonical isoforms
• Glycosylation sites annotated by GlyConnect from a list of publications (PMIDs)
• Glycosylation sites from GlyConnect
• Gold binary interactions with other human proteins
• Human pathways in which at least one protein is mitochondrial GOLD
• Interaction annotations from SwissProt
• KEGG and Reactome pathway names
• Large regions of PE1 proteins that have no associated peptide, and their positional annotations
• PDB structures spanning the complete sequence of the mature protein
• PE1 entries that comply with HPP guidelines (at least 2 non overlapping peptides of at least 9aa from a single data source)
• PE1 entries that do not comply with HPP guidelines (at least 2 non overlapping peptides of at least 9aa from a single data source)
• Pathway names
• Pathways in which SCN1A GOLD interactants are involved
• Peptides that are potential neo N-termini from undescribed isoforms
• Phosphorylation sites from PeptideAtlas Phosphoproteome
• Polymorphisms located on ACE2 and TMPRSS2 and affecting proteins’ activity, structure, PTM...
• Protein domains or regions that frequently occur in interaction mappings
• Protein kinases involved in cancer pathways according to wikipathways
• Protein kinases which are high-confidence drug targets according to CHEMBL
• Protein pairs with at least 50 common interactors (excluding keratins)
• Protein that have a signal sequence which is not cleaved
• Proteins anchored to the membrane via a GPI-anchor
• Proteins annotated as glycosylated but with no recorded glycosylation site
• Proteins associated with a disease but without a disease-causing amino-acid substitution variant
• Proteins associated with an incomplete EC number, no function annotated and high expression in liver
• Proteins associated with diseases that are associated with cardiovascular aspects
• Proteins belonging to Rett syndrome pathways, and their subcellular locations (GOLD)
• Proteins belonging to a family with at least two members in the human proteome
• Proteins binding estradiol and/or similar molecules (similarity search with SMILES), and their associated GO_MF terms
• Proteins binding estradiol and/or similar molecules (substructure search with SMILES) and their associated GO_MF terms
• Proteins entries from a list of gene names
• Proteins expressed in brain with observed IHC expression "high" but not expressed in testis
• Proteins expressed in liver and that have COSMIC variants associated with liver/hepatic cancer
• Proteins for which an interaction mapping region is described for both interactants
• Proteins for which different splice isoforms have a different subcellular location or function
• Proteins for which none of the reported proteotypic peptides is from PeptideAtlas nor MassIVE
• Proteins having at least 2 proteotypic peptides of 7 or 8aa but no proteotypic peptide >= 9 aa
• Proteins interacting with at least 10 members of a protein family
• Proteins interacting with at least one protein which is located in the mitochondrion
• Proteins interacting with small molecules according to DrugBank
• Proteins involved in both Wnt and Hippo signaling pathways
• Proteins involved in coronaviruses/SARS-CoV-2 pathways with associated medical information
• Proteins involved in diseases due to intronic variants with one selected publication
• Proteins involved in diseases with clinical manifestations that include long organs
• Proteins involved in the Reactome pathway "Respiratory electron transport"
• Proteins larger than 1000 amino acids that are located in the nucleus and expressed in the nervous system
• Proteins located in mitochondrion and that lack a transit peptide
• Proteins located in nucleus and nowhere else
• Proteins located in the mitochondrion and which are enzymes
• Proteins located on chromosome 2 and having at least one variant in a phosphorylated tyrosine
• Proteins located on chromosome MT (mitochondrial) coded by a gene located on the plus strand
• Proteins phosphorylated and located in the cytoplasm
• Proteins secreted but without a signal sequence
• Proteins that act as transporters and their TCDB numbers
• Proteins that are PE>1 with at least one proteotypic peptide of at least 9 amino-acids identified in a human sample
• Proteins that are acetylated and methylated and located in the nucleus
• Proteins that are annotated with GO "F" (function) terms prefixed by "Not"
• Proteins that are cytoplasmic with alternate O-glycosylation or phosphorylation at the same positions
• Proteins that are enzymes and with at least one mutagenesis site that decrease or abolish activity
• Proteins that are enzymes requiring the cofactor Ca(2+) and with a calcium binding site
• Proteins that are expressed in liver and involved in transport
• Proteins that are expressed only in liver
• Proteins that are glycosylated and are not located in the membrane
• Proteins that are glycosylated and phosphorylated on an extracellular topological domain
• Proteins that are involved in transport and located in a membrane and that are not glycosylated (experimentally or predicted)
• Proteins that are lipoproteins
• Proteins that are located in both the nucleus and in the cytoplasm
• Proteins that are located in the mitochondrion with an experimental evidence originating not from HPA or DKFZ-GFP
• Proteins that are oxidoreductases and that bind to NAD/NADP
• Proteins that bind RNA but do not contain a RRM domain
• Proteins that bind a metal and are secreted
• Proteins that bind zinc and are not oxidoreductase and not involved in transcription
• Proteins that contains a sequence "QHP" where the Proline is amidated
• Proteins that do not have a cross-reference to an Ensembl ENSG
• Proteins that have a 3D structure in PDB that overlap by at least 50 amino acids with a SH3 domain
• Proteins that have a MS-identified proteotypic peptide that maps partly or fully into a signal sequence
• Proteins that have a protein kinase domain but lack protein kinase activity
• Proteins that have an interaction mapping region that contains the sequence 'ERLI'
• Proteins that have at least one 3D structure solved by NMR
• Proteins that have at least one RRM RNA-binding domain and either no GO "RNA binding" or a GO "RNA binding" with evidence cv:ECO_0000501 or cv:ECO_0000250
• Proteins that have more than one catalytic activity
• Proteins that interact with protein RBM17 and that are involved in splicing
• Proteins that interact with viral proteins
• Proteins that potentially interact with Class I PDZ domains (whose C-terminal sequence has a PDZ-binding consensus)
• Proteins which are "Receptor binding" according to GO and have the Swiss-Prot keyword "Immunity"
• Proteins which are enzymes and that have an incomplete EC number
• Proteins which are enzymes catalyzing a reaction involving lipids
• Proteins which are expressed in liver according to IHC data but not found in HUPO liver proteome set
• Proteins which are involved in cell adhesion according to GO with an evidence which is not used in automatic assertion nor a sequence similarity evidence used in manual assertion
• Proteins which are ion channels and are associated with a disease
• Proteins which are linked to a disease and that do not have orthologs/paralogs in mouse
• Proteins which are located in mitochondrion and have at least one HPA antibody and exist in at least one proteome identification sets
• Proteins which are located on the genome next to a protein which is involved in spermatogenesis
• Proteins which are targets of antipsychotic drugs and highly expressed in brain
• Proteins which are targets of drugs for cardiac therapy
• Proteins which are the substrate of protein kinase SYK
• Proteins which have at least one 3D structure that spans the complete sequence of the mature protein
• Proteins which have at least one zinc finger of any subtype
• Proteins which have been detected in the HUPO liver proteome set but not the HUPO plasma proteome set
• Proteins which have been identified in at least one proteomics set and that are secreted
• Proteins which have genes of length greater than 2 million bp
• Proteins which have one or more negatively charged residue in a transmembrane domain
• Proteins which include a mature chain of less or equal to 50 amino acid residues whose C-terminus is amidated
• Proteins whose gene is annotated to be induced by interferons
• Proteins whose gene is located in chromosome 2 that belongs to families with at least 5 members in the human proteome
• Proteins whose gene is on chromosome 21 with at least one disease annotation from Orphanet
• Proteins whose genes are less than 50000 bp away from the location of the gene coding for protein p53
• Proteins whose genes are located in chromosome 2 region from 2p12 to 2p24.2
• Proteins whose genes are on chromosome 13 and are associated with a disease
• Proteins whose genes are on chromosome 18 and that are experimentally (cv:ECO_0000269) known to be glycosylated or phosphorylated or acetylated
• Proteins whose genes are on chromosome 21 that have "gold" variants not associated with a disease
• Proteins whose genes are on chromosome X and which do not have a ortholog in mouse
• Proteins whose sequence was updated in 2014
• Proteins with 10 or more gold interactions with SH3 domain-containing proteins
• Proteins with 3 disulfide bonds and that are not annotated as hormones
• Proteins with 7 transmembrane regions
• Proteins with ENV polyprotein domains and matching viral species
• Proteins with Poly-Proline stretches
• Proteins with RNA-seq observed expression "not detected" and IHC observed expression : "high" (same tissue or children)
• Proteins with a 3D structure in complex with another human protein which is not reported as binary interactant
• Proteins with a DNA-binding region but not located in the nucleus
• Proteins with a PDZ domain that interact with at least one protein which is expressed in brain
• Proteins with a coiled coil region and that are involved in transcription but do not contain a bZIP domain
• Proteins with a cross-reference to CCDS
• Proteins with a gene alternative name starting with IL27
• Proteins with a gene name that starts with "CLDN"
• Proteins with a mature chain of from 300 to 400 residues
• Proteins with a mature chain of less than 1000 amino acids which are secreted and do not contain cysteines in the mature chain
• Proteins with a mitochondrial transit peptide
• Proteins with a molecular weight less than 25 kDa
• Proteins with a mutagenesis in a position that correspond to an annotated active site
• Proteins with a propeptide
• Proteins with a protein existence "At transcript level" (PE=2)
• Proteins with a protein existence not "At protein level" (PE=1) and with a HGNC approved gene name that contains "orf"
• Proteins with a sequence containing "FF.+QYE" where ".+" is any peptide of any length
• Proteins with a sequence containing "FF.QYE" where "." is any single amino-acid
• Proteins with a sequence that does not contain a lysine in the mature region
• Proteins with a signal sequence
• Proteins with a variant having an impact on a binary interaction
• Proteins with a variant having an impact on the nucleus localization at level GOLD
• Proteins with alternative acetylation or Ubl conjugation (SUMO or Ubiquitin) at the same positions
• Proteins with an active site that is a proton acceptor
• Proteins with an interaction mapping encompassing a coiled coil or bZip region
• Proteins with associated cancer pathways in WikiPathways (via Disease Ontology classification)
• Proteins with associated pathways in WikiPathways
• Proteins with at least 2 HPA antibodies whose genes are located on chromosome 21 and that are highly expressed according to IHC in heart
• [Proteins with at least 2 validating peptides >=9aa found in blood plasma, urine or cerebrospinal fluid (criteria for biomarker)].](/sparql-examples/examples/neXtProt/NXQ_00226.html)
• Proteins with at least 50 interactors that are not associated with a disease
• Proteins with at least one 3D structure and that are located in the mitochondrion and are linked with a disease
• Proteins with at least one 3D structure and that are phosphorylated
• Proteins with at least one 3D structure of resolution less than 3 Angstroms
• Proteins with at least one HPA antibody that are located in the peroxisome
• Proteins with at least one annotated mutagenesis site
• Proteins with at least one cross-reference to SMR (Swiss Model Repository) but no cross-references to PDB
• Proteins with at least one homeobox domain and with at least one variant in the homeobox domain(s)
• Proteins with at least one known SUMOylation site
• Proteins with at least one phosphotyrosine but no phosphoserine or phosphothreonine
• Proteins with at least one proteotypic peptide 9aa+ not mapping on canonical isoform
• Proteins with at least one selenocysteine in their sequence
• Proteins with at least one sequence variant
• Proteins with at least one somatic variant
• Proteins with at least one variant of the type "C->X" (Cys to anything else) that are linked to one or more diseases
• Proteins with at least one variant of the types "A->R" or "R->A"
• Proteins with at least two antibodies available from Human Protein Atlas that have associated tissue expression annotations from immunohistochemistry studies
• Proteins with at least two proteotypic synthetic peptides from SRMAtlas of at least 9 amino acids in length
• Proteins with both RNA-seq expression and observed IHC expression "high" in brain or one of its subparts
• Proteins with cross-references to InterPro
• Proteins with experimentally determined lengthy alpha-helices (> 75 aa)
• Proteins with from 2 to 4 transmembrane regions
• Proteins with high proline content
• Proteins with high-frequency missense variants involved in bacterial infection, with dbSNP identifiers and position on the canonical isoform
• Proteins with interactions obtained from x-ray crystallography
• Proteins with more than 10 alternative isoforms
• Proteins with more than 10 reported interactions (of 'gold' quality)
• Proteins with more than 12 WD repeats (ie: with at least two beta-propellers)
• Proteins with no function annotated
• Proteins with no known (annotated) domain
• Proteins with no reported mitochondrial localization but interacting with 20 or more mitochondrial proteins ('gold' quality)
• Proteins with one SH2 and two SH3 domains
• Proteins with one or more glycosylation sites reported in PubMed:20570859 or PubMed:14760718
• Proteins with one transmembrane domain and no annotated topology
• Proteins with protein existence "At protein level" (PE=1) and at least one proteotypic peptide
• Proteins with protein existence "At protein level" (PE=1) that have no function annotated, are highly expressed in brain and have homologs in Drosophila melanogaster according to OrthoDB
• Proteins with protein existence "At transcript level" (PE=2) or "Inferred from homology" (PE=3) or "Predicted" (PE=4)
• Proteins with sequences 100% identical to other proteins, grouped
• Proteins with sequences 100% identical to other proteins
• Proteins with subcellular location gold and list of all these locations
• Query for an Entry Instance e.g entry:NX_Q8WZ42
• Query for an Isoform Instance e.g isoform:NX_Q8WZ42-1
• Recommended isoform names for MSH6
• References for SCN1A variants causing Dravet syndrome
• Retrieve all positional annotations at a given position on a neXtProt isoform
• Secreted proteins that have at least one PTM in a position of a variant
• Terms of controlled vocabularies containing some word(s)
• Transmembrane proteins with at least 100 consecutive aa located in the EXTRACELLULAR OR LUMENAL compartment.
• Transmembrane proteins with the number of transmembrane domains in the canonical isoform by decreasing order
• UniPathway and related GO
• Variants identified in exome datasets in a frequent homozygote state
• Variants identified in exome datasets with a frequency of >0.1
• Variants in MECP2 causing Rett Syndrome
• Variants with normal "ubiquitin-protein transferase activity" and decreased or increased binding to UBE2D1 (example: BRCA1-p.Ile89Thr)
• Variants with phenotype annotation that map to a 3D structure
• What are the 25 most frequent families with member count